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VascuLife™ VEGF-Mv Cell Culture Medium

VascuLife VEGF-Mv (containing Vascular Endothelial Growth Factor) is a new medium optimized for the culture of human microvascular endothelial cells. VascuLife supports the rapid proliferation of these cells in a 5% serum environment. VascuLife contains no antimicrobials and no phenol red; components that can cause cell stress and “masking effects” that may influence experimental results (Lifeline® offers these traditional supplements; however these are not needed, or recommended, to achieve optimal cell performance). VascuLife VEGF-Mv contains rh VEGF which may affect stimulation assays. Lifeline recommends using VascuLife EnGS-Mv for these studies.

Cat-No.: Product Description Size
LL-0005 VascuLife VEGF-Mv Cell Culture Medium Kit (VascuLife Basal Medium, LifeFactors Kit) Kit
LM-0002 VascuLife Basal Medium 475 ml
LS-1029 VascuLife VEGF-Mv LifeFactors Kit Kit

VascuLife Culture Medium Components

VascuLife is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors™.” This kit allows you to prepare fresh medium in your laboratory, extending shelf-life and
enhancing performance. VascuLife Basal Medium comes in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

Quality Testing for Guaranteed Consistency and Reproducible Results

Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style
quality assurance in every production run. Exacting standards and production procedures ensure lot-to-lot consistency. Every production lot of media is extensively tested using normal human cells.

• Sterility Testing Negative for bacteria and fungal growth

• Osmolality 270 +/- 10 mOsm

• pH 7.8 +/- 0.3

• Endotoxin < 0.5 EU/mL

• Cell Testing Rate of proliferation and morphology

VascuLife® VEGF-Mv medium grows human dermal microvascular endothelial cells and other human microvascular endothelial cells at rates that meet or exceed other commercially available serum-containing medium while maintaining excellent cell morphology. In comparisons with commercially available medium, VascuLife shows superior proliferation at different seeding densities.

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria.
If Lifeline’s products do not meet your expectations or our posted performance and quality standards,
we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific
QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty
statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Innovative Packaging Features
Lifeline has made significant improvements to traditional medium packaging:
• The specially designed shrink label works as a light barrier to protect the medium from light damage.
• A temperature gauge, built into the package, assists in contamination-free medium warming.
• A protective sleeve helps protect the medium from contaminants found in the water bath.
• Lifeline is an environmentally friendly company. Our cell culture media bottles are recyclable, and we ship
all products with biodegradable packing material.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for
use in in vitro diagnostics or clinical procedures.

CellSystems Biotechnologie Vertrieb GmbH | Langeler Ring 5 | D - 53842 Troisdorf | Phone +49 (0) 2241 25515 - 0 | Fax +49 (0) 2241 25515 - 30