VascuLife® VEGF medium grows HUVEC and other human large-vessel endothelial cells at rates that meet or exceed commercially available serum-containing medium while maintaining excellent cell morphology. In comparisons with commercially available medium, VascuLife VEGF shows superior proliferation at different seeding densities. (HUVEC, P2, 5 days after inoculation, 100X)
Quality Testing for Guaranteed Consistency and Reproducible Results Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style • Sterility Testing Negative for bacteria and fungal growth • Osmolality 270 +/- 10 mOsm • pH 7.8 +/- 0.3 • Endotoxin < 0.5 EU/mL • Cell Testing Rate of proliferation and morphology
VascuLife Culture Medium Components VascuLife is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf-life and enhancing performance. VascuLife Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage. VascuLife VEGF Culture Medium VascuLife VEGF (containing Vascular Endothelial Growth Factor) is a new low serum medium optimized for the rapid proliferation of human endothelial cells including Human Umbilical Vein Endothelial Cells (HUVEC), aortic endothelial cells and other human large-vessel endothelial cells. VascuLife supports the growth of these cells in a low serum environment. VascuLife contains no antimicrobials and no phenol red; components that can cause cell stress and “masking effects” that may influence experimental results (Lifeline® offers these traditional supplements; however these are not needed, or recommended, to achieve optimal cell performance). VascuLife VEGF contains rh VEGF, which may affect stimulation assays especially where VEGF is the primary stimulator. Lifelife recommends using VascuLife EnGS for these types of studies.
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The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information. Innovative Packaging Features Safety Statement
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