Lifeline’s Renal Epithelial Cells are cryopreserved after primary or secondary culture to ensure the highest viability and plating efficiency. Our Renal Epithelial Cells are quality tested in RenaLife Medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Lifeline’s Renal Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in RenaLife Medium; an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline offers these traditional supplements; however they are not recommended to achieve optimal cell performance in most research applications.

Cell Features:

Mixed Renal Epithelial, Renal Cortical Epithelial, and Renal Medullary Epithelial are cryopreserved after primary culture; isolated from human kidney tissue and expanded once before cryopreservation.

Renal Proximal Tubule Epithelial are cryopreserved after secondary culture; isolated from human kidney tissue and expanded twice before cryopreservation.

All Renal Epithelial Cell types can be grown in a 0.5% serum medium without phenol red or antimicrobials when cultured in RenaLife Medium.

All Renal Epithelial Cell types are extensively tested for quality and optimal performance.

Lifeline guarantees performance and quality.

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Cat-No.:	Product Description	Size
FC-0017	Mixed Renal Epithelial Cells	500,000 cells/amp
FC-0012	Renal Cortical Epithelial Cells	500,000 cells/amp
FC-0018	Renal Medullary Epithelial Cells	500,000 cells/amp
FC-0013	Renal Proximal Tubule Epithelial Cells	500,000 cells/amp
LL-0025	RenaLife™ Medium Complete Kit	Kit

Cell Count	500,000 cryopreserved cells per ampule
0,5% Serum Proliferation	Population growth at various densities
Morphology	Normal cell appearance for 15 population doublings
Cell Viabilty	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for bacteria and fungal growth for 14 days
Virus Testing	Negative for HIV, HBV, and HCV by PCR
Specific Enzyme Test	Renal Proximal Tubule cells have g-glutamyl transferase activity

NORMAL HUMAN RENAL EPITHELIAL CELLS ARE TESTED FOR: