Cat-No.:	Product Description	Size
LL-0025	RenaLife Cell Culture Medium Kit (RenaLife Basal Medium, LifeFactors Kit)	Kit
LM-0010	RenaLife Basal Medium	475 ml
LS-1048	RenaLife LifeFactors Kit	Kit

RenaLife Culture Medium Components

RenaLife is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors^®” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. RenaLife Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

Quality Testing for Guaranteed Consistency and Reproducible Results

Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style
quality assurance in every production run. Exacting standards and production procedures ensure lot-to-lot consistency. Every production lot of media is extensively tested using normal human cells.

• Sterility Testing Negative for bacteria and fungal growth

• Osmolality 315 +/- 10 mOsm

• pH 7.5 +/- 0.2

• Endotoxin < 0.5 EU/mL

• Cell Testing Rate of proliferation and morphology

RenaLife™  Medium grows human renal epithelial cells through at least 15 population doublings at rates that are comparable to other commercially available low-serum medium while maintaining more characteristic epithelial morphology, e.g. more compact cuboidal shape, high refractive index and clarity of the cytoplasm. (Renal Cortical Epithelial Cells, 3^rd subculture, 100X)

g-Glutamyltransferase (GGT) assay:

Incubated for 30 min at 37°C.
Substrate – g-glutamyl-p-nitroanilide
Acceptor – glycylglycine
Product – p-nitroaniline (PNA)

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria.
If Lifeline’s products do not meet your expectations or our posted performance and quality standards,
we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific
QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty
statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Innovative Packaging Features
Lifeline has made significant improvements to traditional medium packaging:
• The specially designed shrink label works as a light barrier to protect the medium from light damage.
• A temperature gauge, built into the package, assists in contamination-free medium warming.
• A protective sleeve helps protect the medium from contaminants found in the water bath.
• Lifeline is an environmentally friendly company. Our cell culture media bottles are recyclable, and we ship
all products with biodegradable packing material.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for
use in in vitro diagnostics or clinical procedures.