Cell Features:

HEM are cryopreserved after primary culture or secondary culture; isolated from human skin and expanded once or twice in culture vessels before being harvested for cryopreservation.

HEMn can be grown in a low serum medium without phenol res or antimicrobials when cultured in DermaLife m Medium

HEMa can be grown in a low serum medium without phenol red or antimicrobials when cultured in DermaLife Ma Medium.

Culture HEM without PMA or Cholera toxin or other artificial non-specific stimulants.

Melanocytes are extensively tested and guaranteed for quality and optimal performance

Lifeline guarantees performance and quality.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Normal Human Epidermal Melanocytes are tested for:

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Lifeline’s HEM are cryopreserved as primary or secondary cells* to ensure the highest viability and plating efficiency. Our HEMn are quality tested in DermaLife M Medium and our HEMa are quality tested in DermaLife Ma Medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than media supplemented with higher serum, PMA or Cholera toxin. Lifeline’s HEM need not be exposed to antimicrobials or phenol red when cultured in DermaLife M or DermaLife MA Medium; an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline offers these traditional supplements; however they are not recommended to achieve optimal cell performance in most research applications.

Cell Count	500,000 cryopreserved cells per ampule
Low Serum Proliferation	Population growth at various densities
Morphology	Normal cell appearence for 15 population doublings (HEMn) or 10 population doublings (HEMa)
Cell Viability	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for bacteria and fungal growth for 14 days
Virus Testing	Negative for HIV, HBV and HCV by PCR
Specific Testing	L- Dopa Oxidase Activity Assay at high passage

Cat-No.:	Product Description	Size
FC-0023	HEMn, Normal Human Epidermal Melanocytes, Primary	500,000 cells/amp
FC-0019	HEMn, Normal Human Epidermal Melanocytes, Secondary	500,000 cells/amp
LL-0027	Dermalife M Complete Medium Kit	Kit
FC-0030	HEMa, Normal Human Epidermal Melanocytes, Secondary	500,000 cells/amp
LL-0039	DermaLife Ma Complete Medium Kit	Kit