Cat-No.:	Product Description	Size
FC-0007	Cryopreserved Normal Human Epidermal Keratinocytes, Neonatal	500,000 cells/amp
FC-0025	Cryopreserved Normal Human Epidermal Keratinocytes, Adult	500,000 cells/amp
LL-0007	Dermalife K Complete Medium Kit	Kit
LL-0029	Dermalife K Calcium-Free Complete Medium Kit	Kit

Cell Features:
HEKn are cryopreserved after primary culture; e.g. cells are isolated from neonatal human foreskin and expanded
once before cryopreservation.

HEKa are cryopreserved after primary culture; e.g. cells are isolated from adult human skin and expanded
once before cryopreservation.

HEK can be grown without serum, phenol red or antimicrobials when cultured in DermaLife medium..

HEK are extensively tested and guaranteed for quality and optimal performance

Lifeline guarantees performance and quality.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style
quality assurance in every production run. Exacting standards and production procedures ensure lot-to-lot consistency. Every production lot of Dermalife K Medium is extensively tested using normal human keratiniocytes cells.

Normal Human Dermal Keratinocytes are tested in serum free DermaLife Medium for:

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Lifeline’s HEK are cryopreserved after primary culture to ensure the highest viability and plating efficiency. Our HEK are quality tested in DermaLife K Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented medium. Lifeline’s HEK need not be exposed to antimicrobials or phenol red when cultured in DermaLife medium; an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline offers these traditional supplements; however they are not recommended to achieve optimal cell performance in most research applications.

Cell Count	500,000 cryopreserved cells per ampule
Serum Free Proliferation	Population growth at various densities
Morphology	Normal cell appearence for 15 population doublings
Cell Viability	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for bacteria and fungal growth for 14 days
Virus Testing	Negative for HIV, HBV and HCV by PCR