FibroLife Serum Free Culture Medium FibroLife is a new defined medium optimized for the serum-free culture of fibroblasts. FibroLife supports the growth of these cells in a serum-free environment at rates equal to or greater than media supplemented with 2-10% FBS (see data on page two). FibroLife contains no serum, no antimicrobials and no phenol red; components that can cause cell stress and “masking effects” that may influence experimental results (Lifeline offers these traditional supplements; however these are not needed, or recommended, to achieve optimal cell performance.) FibroLife provides a perfect cell system to establish serum-free human feeder layers for stem cell culture or as a model to study wound healing, toxicology or basic cell biology.
FibroLife Serum Free Culture Medium Components FibroLife is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. FibroLife Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage. Quality Testing for Guaranteed Consistency and Reproducible Results Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style • Sterility Testing Negative for bacteria and fungal growth • Osmolality 270 +/- 10 mOsm • pH 7.4 +/- 0.2 • Endotoxin < 0.5 EU/mL • Cell Testing Rate of proliferation and morphology
FibroLife® Medium grows human fibroblasts at rates that meet or exceed commercially available serum-containing and serum-free media while maintaining excellent cell morphology.
The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information. Innovative Packaging Features Safety Statement   
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