FibroLife S2 Culture Medium FibroLife S2 is a variation of our FibroLife cell culture medium optimized for low serum (2%) culture of fibroblasts. FibroLife supports the growth of these cells in a 2% serum environment at rates equal to or greater than comparable media supplemented with 2-10% FBS. FibroLife S2 contains no antimicrobials and no phenol red, components that can cause cell stress and “masking effects” that may influence experimental results (Lifeline® offers these traditional supplements; however these are not needed, or recommended, to achieve optimal cell performance). FibroLife S2 provides a perfect cell system to establish low serum human feeder layers for stem sell culture or as a model to study wound healing, toxicology or basic cell biology.
FibroLife S2 Culture Medium Components FibroLife S2 is offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. FibroLife Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors (growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage. Quality Testing for Guaranteed Consistency and Reproducible Results Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style • Sterility Testing Negative for bacteria and fungal growth • Osmolality 270 +/- 10 mOsm • pH 7.4 +/- 0.2 • Endotoxin < 0.5 EU/mL • Cell Testing Rate of proliferation and morphology
FibroLife® S2 medium grows fibroblasts in low serum conditions at rates that meet or exceed commercially available serum-containing medium while maintaining excellent cell morphology. In comparisons with commercially available medium, FibroLife S2 medium shows superior proliferation at different seeding densities.
The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information. Innovative Packaging Features Safety Statement    
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