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Normal Human Airway Epithelial Cells

Lifeline’s normal Human Airway Epithelial Cells, when grown in Lifeline’s BronchiaLife TM Medium, provide an ideal serum-free culture model, for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing.

Cat-No.: Product Description Size
FC-0035 HBTEC, Normal Human Bronchial/Tracheal Epithelial Cells, Primary 500,000 cells/amp
FC-0011 HBTEC, Normal Human Bronchial/Tracheal Epithelial Cells, Secondary 500,000 cells/amp
LL-0023 BronchiaLifeTM B/T Medium Complete Kit Kit
FC-0016 HSAEC, Normal Human Small Airway Epithelial Cells, Primary 500,000 cells/amp
LL-0037 BronchiaLifeTM SAE Medium Complete Kit Kit

Cell Features:

HBTEC are cryopreserved as primary or secondary cells, e.g. cells are isolated from human bronchi/trachea and expanded once or twice in culture vessels before being harvested for cryopreservation.

HSAEC are cryopreserved after primary culture, e.g. cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.

Lifeline’s Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.

Human Airway Epithelial Cells are extensively tested for quality and optimal performance

Lifeline guarantees performance and quality.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style
quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Normal Human Airway Epithelial Cells are tested for:
The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Lifeline’s HBTEC and HSAEC are cultured without retinoic acid and cryopreserved as primary or secondary cells* to ensure the highest viability and plating efficiency. Our HBTEC are quality tested in BronchiaLife B/T Medium and our HSAEC are quality tested in BronchiaLife SAE Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media. Lifeline’s Human Airway Epithelial Cells are not exposed to antimicrobials or phenol red when cultured in BronchiaLife medium; an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline offers these traditional supplements; however they are not needed, or recommended, to achieve optimal cell performance in most research applications.

Cell Count 500,000 cryopreserved cells per vial
Proliferation Population growth at various densities
Morphology Normal cell appearence for 15 population doublings
Cell Viability Minimum 50% viability when thawed from cryopreservation
Sterility Testing Negative for mycoplasma, bacteria and fungal growth for 14 days
Virus Testing Negative for HIV, HBV and HCV by PCR

CellSystems Biotechnologie Vertrieb GmbH | Langeler Ring 5 | D - 53842 Troisdorf | Phone +49 (0) 2241 25515 - 0 | Fax +49 (0) 2241 25515 - 30