MCFibs are quality tested in FibroLife® Serum-Free Medium to ensure high viability and plating efficiency. MCFibs are cultured in FibroLife Serum-Free Medium without exposure to undefined serum, antimicrobials, or phenol red, removing the negative impact that these supplements may have on experimental results. MCFibs are ready for use and may be plated directly onto gelatin coated culture vessels. Lifeline’s MCFibs also reduce the need for researchers to store toxic mitomycin C in their laboratory.

Cell Features:

Cryopreserved in second passage (P₂).

FMCFibs remain viable and attached in serum-free, animal protein-free, phenol red-free, and antimicrobial-free medium.

MCFibs are extensively tested for quality and optimal performance.

MCFibs are ready to use.

MCFibs are pretreated to help reduce toxic agents in your lab.

Lifeline guarantees performance and quality.

MCFibs are tested for suitability as feeder layers for human embryonic stem cell cultures.

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Cat-No.:	Product Description	Size
FC-0002	MCFibs™, Mitomycin C Treated Normal Human Fibroblasts	3,000,000 cells/amp
LL-0001	FibroLife® Serum-Free Medium Complete Kit	Kit
LL-0011	FibroLife® S2 Medium Complete Kit	Kit

Cell Count	3,000,000 cryopreserved cells per ampoule
Feeder Layer	MCFibs are used to culture embryonic stem cells for two passages to qualify their use as feeder layers.
Serum-Free	MCFibs are cultured in FibroLife Serum-Free Medium to qualify their use as feeder layers in a serum-free system.
Morphology	Normal cell appearance
Cell Viability	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for mycoplasma, bacteria and fungal growth
Virus Testing	Negative for HIV, HBV and HCV by PCR

MCFibs are tested for: