Lifeline´s HAoSMC are cryopreserved in secound passage to ensure the highest viability an plating efficiency. Our HAoSMC are quality tested in VascuLife SMC Medium to ensure optimal low serum growth over a peroid of at least 15 population doublings at rates equal to or greater than other serum-supplemented media. Lifeline´s HAoSMC are not exposed to antimicrobials or phenol red when cultured in VascuLife SMC Medium; an advantage since these supplements can cause cell stress and "making effects" that may negatively impact experimental results. Lifeline offers these traditional supplements; however they are not recommended to achieve optimal cell performance in most research applications

Cell Features:

HAoSMC isolated from human aortic tissue are cultured for two passage (P2) before cryopreservation.

HAoSMC can be grown without phenol red or antimicrobials when cultured in VascuLife SMC Medium.

HAoSMC are extensively tested for quality and optimal performance.

Lifeline guarantees performance and quality.

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Cat-No.:	Product Description	Size
FC-0015	HAoSMC, Normal Human Aortic Smooth Muscle Cells	500,000 cells/amp
LL-0014	VascuLife SMC Complete Medium Kit	Kit

Cell Count	500,000 cryopreserved cells per ampule
5% Serum Proliferation	Population growth at various densities
Morphology	Normal cell appearence for 15 population doublings
Cell Viability	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for bacteria and fungal growth for 14 days
Virus Testing	Negative for HIV, HBV and HCV by PCR
Specific Staining	Factor VIII negatice Smooth muscle a-actin positive after differentiation

Normal Human Aortic Smooth Muscle Cells are tested for: