Cat-No.:	Product Description	Size
FC-0014	HAoEC, Normal Human Aortic Endothelial Cells	500,000 cells/amp
LL-0002	VascuLife EnGS Complete Medium Kit	Kit
LL-0003	VascuLife VEGF Complete Medium Kit	Kit

Cell Features:

Cryopreserved in second passage (P₂).

HAoEC can be grown in a 2% serum medium without phenol red or antimicrobials when cultured in VascuLife medium.

Culture HAoEC with or without VEGF.

HAoEC are extensively tested for quality and optimal performance.

Lifeline guarantees performance and quality..

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates ISO style
quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Normal Human Aortic Endothelial Cells are tested for:

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Lifeline’s HAoEC are cryopreserved in second passage to ensure the highest viability and plating efficiency. Our HAoECs are quality tested in VascuLife 2% serum medium to ensure optimal reduced-serum growth over a period of at least 15 population doublings at rates equal to or greater than other serum-supplemented medium. Lifeline’s HAoEC are not exposed to antimicrobials or phenol red when cultured in VascuLife medium; an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline offers these traditional supplements; however they are not recommended to achieve optimal cell performance in most research applications.

Cell Count	500,000 cryopreserved cells per ampule
2% Serum Proliferation	Population growth at various densities
Morphology	Normal cell appearence for 15 population doublings
Cell Viability	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for bacteria and fungal growth for 14 days
Virus Testing	Negative for HIV, HBV and HCV by PCR
Specific Staining	Factor VIII positive Smooth muscle a-actin negative