Lifeline’s Fibroblasts are cryopreserved after primary culture to ensure the highest viability and plating efficiency. Our fibroblasts are quality tested in both FibroLife® Serum-Free Medium and FibroLife S2 Medium to ensure optimal growth at rates equal to or greater than classical serum-supplemented medium. Lifeline’s fibroblasts need not be exposed to undefined serum, antimicrobials or phenol red when cultured in FibroLife Serum-Free Medium; an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results. Lifeline® offers these traditional supplements; however they are not recommended to achieve optimal cell performance in most research applications.

Cell Features:
HDFn are cryopreserved after primary culture, cells are isolated from neonatal human foreskin and expanded once before cryopreservation.

HDFa are cryopreserved after primary culture, cells are isolated from adult human foreskin and expanded once before cryopreservation.

Fibroblasts can be grown in serum-free, animal protein-free, phenol red-free, and antimicrobial free conditions when cultured in FibroLife Serum-Free Medium.

Fibroblasts are extensively tested and guaranteed for quality and optimal performance.

Lifeline’s Fibroblasts are suitable for use as feeder layers for human embryonic stem cell cultures and other cell culture applications requiring feeder layers.

The Lifeline Guarantee
Lifeline’s rigorous quality control ensures sterility and performance to standardized testing criteria. If Lifeline’s products do not meet your expectations or our posted performance and quality standards, we will replace them at no charge or provide a full refund. Upon request, Lifeline will provide lot-specific QC test results, material safety data sheets and certificates of analysis. See complete guarantee/warranty statement at lifelinecelltech.com or contact your Lifeline representative for more information.

Safety Statement
This product is for research use only. This product is not approved for human or veterinary use, or for use in in vitro diagnostics or clinical procedures.

Quality Testing for Guaranteed Consistency and Reproducible Results
Lifeline Cell Technology manufactures products using the highest quality raw materials and incorporates extensive quality assurance in every production run. Exacting standards and production procedures ensure consistent performance.

Cat-No.:	Product Description	Size
FC-0001	HDFn, Normal Human Fibroblasts-neonatal	500,000 cells/amp
FC-0002	MCFibs™, Mitomycin C Treated Normal Human Fibroblasts	3,000,000 cells/amp
FC-0024	HDFn, Normal Human Fibroblasts-adult	500,000 cells/amp
LL-0001	FibroLife® Serum-Free Medium Complete Kit	Kit
LL-0011	FibroLife® S2 Medium Complete Kit	Kit

Cell Count	500,000 cryopreserved cells per ampule
Proliferation	Population growth at various densities
Morphology	Normal cell appearance at: HDFn: 10 population doublings in serum-free medium HDFa: 2 pasages post thaw in serum-free medium. Both HDFn and HDFa:15 population doublings in 2% serum medium.
Cell Viabilty	Minimum 50% viability when thawed from cryopreservation
Sterility Testing	Negative for mycoplasma, bacteria and fungal growth
Virus Testing	Negative for HIV, HBV and HCV by PCR

Normal HDFn and HDFa are tested in both serum-free and 2% serum FibroLife Medium for: