The morphology of Epidermal Skin Test EST1000 is divided into a macroscopic and a microscopic view.

The epidermis models are cultivated in cell culture inserts with 0.6 cm² surface area. The membranes are covered completely by the cells and show a matt white, dry surface after complete differentiation. Detached from the membrane by treatment with dispase EST1000 withstands even mechanical stress.

The cross section of the model reveals the cellular structure of EST1000 which resembles perfectly that of natural epidermis:

Palisade-like cells of the basal layer (A) are followed by cells which are round within the stratum spinosum (B) and flatten to the stratum granulosum (C). Final differentiation leads to the intact barrier of the stratum corneum (D).

The physiological composition of lipids in the epidermis is a prerequisite for the formation of a functional barrier. In comparison to explanted human skin EST1000 shows the same spectrum of polar and unpolar lipids.