CellSystems^® EST1000 is a 3 D reconstructed human epidermis model. It consists of normal primary human epidermal keratinocytes from one neonatal donor. During production a submerse culture of the keratinocytes is followed by a culture at air-liquid interphase. This “airlift culture” under defined media conditions stimulates the differentiation of the cells and the generation of the physiological epidermal layers. The cellular structure of EST1000 resembles that of the fully differentiated natural epidermis showing a base membrane, proliferating keratinocytes and a stratum corneum with an intact barrier function.

EST1000 has been developed to meet the strict criteria for regulatory toxicology in vitro (eg. skin corrosion testing).

For research applications EST1000 is available in different maturation stages and produced under custom specific conditions (eg. w/o hydrocortisone) on request. It is a robust epidermal skin model and it can be cultured for up to 3-4 weeks at air-liquid interphase (airlift culture). During this time the viability, and therefore the mitogenic potential of the basal layers are maintained. The thickness of the stratum corneum increases because of the lack of mechanical forces leading to removal of the topmost layers. This allows research in different phases of skin differentiation and maturation.

Customised products on request.