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Worthington

Tissue Dissociation

Trypsin from Worthington Biochemical Corporation*

Trypsin is a pancreatic serine protease with a specificity for peptide bonds involving the carboxyl group of the basic amino acids, arginine and lysine. Trypsin is one of the most highly specific proteases known, although it also exhibits some esterase and amidase activity.

Purified trypsin alone is usually ineffective for tissue dissociation since it shows little selectivity for extracellular proteins. Combinations of purified trypsin and other enzymes such as elastase and/or collagenase have proven effective for dissociation.

* Text is quoted from the Worthington Enzyme Manual

Product Highlight: Trypsin

Product Name Description Activity Quantity Cat.No.
Trypsin, Modified, Sequencing Grade

Code TRSEQZ

 Trypsin, treated with
L-(tosylamido-2-phenyl) ethyl chloromethyl ketone to inhibit contaminating chymotryptic activity, chemically modified to promote stability and further purified to remove autolysis fragments, resulting in a highly stable trypsin product resistant to autolysis while retaining specificity. Source: Bovine Pancreas. Storage Conditions: Store at -20ºC PROTECT FROM MOISTURE. REQUIRES SPECIAL SHIPPING: ICE PACK.		 ≥4 units per mg protein 4 x 25µg

4 x 100 µg

1 mg

Bulk

 LS02120

LS02122

LS02123

LS02124
Trypsin, Purified, Sequenzing Grade II

Code TRSEQII

 Bovine trypsin that has been treated with
L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity and extensively purified to remove autolysis products. Supplied as a lyophilized powder. Source: Bovine Pancreas. Storage Conditions: Store at -20ºC. PROTECT FROM MOISTURE. REQUIRES SPECIAL SHIPPING: ICE PACK.		 ≥150 units per mg protein (at least 8,625 BAEE/2875 USP/NF units per mg protein)

4 x 100 µg

1 mg

Bulk

4x25µg

LS02115

LS02117

LS02119

LS02118
Trypsin,
TPCK Treated

Code TRTPCK

 A chromatographically purified, diafiltered, lyophilized powder that has been treated with
L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)]. Source: Bovine Pancreas. Storage Conditions: Store at
2-8ºC. PROTECT FROM MOISTURE.		 ≥ 180 u per mg protein (≥10,350 BAEE/3,450 USP/NF u/mg protein)

100 mg

500 mg

1 g

Bulk		 LS003740

LS003741

LS003744

LS003742
Trypsin 3X

Code TRL3

 Supplied as a chromatographically purified, diafiltered and lyophilized powder. Source: Bovine Pancreas. Storage Conditions: Store at 2-8ºC. PROTECT FROM MOISTURE. ≥ 180 u per mg protein (≥10,350 BAEE/3,450 USP/NF u/mg protein)

100 mg

1 g

Bulk		 LS003708

LS003707

LS003709
Trypsin 2X

Code TRL

 Supplied as a chromatographically purified, diafiltered and lyophilized powder. TPCK treated. Source: Bovine Pancreas. Storage Conditions: Store at
2-8ºC. PROTECT FROM MOISTURE.		 ≥ 180 u per mg protein (≥10,350 BAEE/3,450 USP/NF u/mg protein)

100 mg

1 g

10 g

Bulk		 LS003702

LS003703

LS003704

LS003706
Trypsin,
0.22µ Filtered

Code TRLS

 Trypsin chromatographically purified, diafiltered (Code TRL3) filtered thorugh a 0.22 micron pore size membrane and lyophilized in sterile vials. This product is not tested for pyrogenicity. Source: Bovine Pancreas. Storage Conditions: Store at
2-8ºC. PROTECT FROM MOISTURE.		 ≥ 180 u per mg protein (≥10,350 BAEE/3,450 USP/NF u/mg protein)

50 mg

5 X 50 mg

Bulk		 LS003736

LS003734

LS003738
Trypsin, 2X, Sterile, Irradiated

Code TRLVMF

 Chromatographically purified (Code: TRL), lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR113.53c. Each vial is filled to contain ≥100 mg. Source: Bovine Pancreas. Storage Conditions: Store at 2-8ºC. ≥ 180 u per mg protein (≥10,350 BAEE/3,450 USP/NF u/mg protein)

100 mg

5 X 50 mg

Bulk		 LS004454

LS004452

LS004458
Trypsin, TPCK-Treated, Irradiated

Code TRTVMF

 Chromatographically purified trypsin treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity according to Kostka, V., and Carpenter, F.H.: JBC, 239, 1799 (1964), Code: TRTPCK, lyophilized, irradiated and tested for the absence of mycoplasma and extraneous virus according to 9 CFR 113.53c. Each vial is filled to contain ≥100 mg. Source: Bovine Pancreas. Storage Conditions: Store at 2-8ºC. PROTECT FROM MOISTURE. ≥ 180 u per mg protein (≥10,350 BAEE/3,450 USP/NF u/mg protein)

100 mg

5 X 50 mg

 LS003750

LS003752

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