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Worthington

Tissue Dissociation

Papain from Worthington Biochemical Corporation *

Papain is a sulfhydryl protease from Carica papaya latex. Papain has wide specificity and it will degrade most protein substrates more extensively than the pancreatic proteases. It also exhibits esterase activity.

With some tissues papain has proved less damaging and more effective than other proteases. Huettner and Baughman (1986) describe a method using papain to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats.

* Text is quoted from the Worthington Enzyme Manual

Product Highlight: Papain

Product Name Description Activity Quantity Cat.No.
Papain, Suspension

Code PAP

Supplied as a 2X crystalline suspension in 50mM sodium acetate,
pH 4.5. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM. Source: Papaya Latex. Storage Conditions: Store at
2-8ºC. REQUIRES SPECIAL SHIPPING: ICE PACK.
Activates to 20 units per mg protein 25 mg

100 mg

1 gm

Bulk

LS003124

LS003126

LS003127

LS003128

Papain, Lyophilized

Code PAPL

Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1mM EDTA, 0.067mM mercaptoethanol and 5.5mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. Source: Papaya Latex. Storage Conditions: Store at
2-8ºC.
Activates to 15 units per mg protein

25 mg

100 mg

1 gm

Bulk

LS003118

LS003119

LS003120

LS003122

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