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Papain Dissociation System from Worthington Biochemical Corp.*
Proteolytic enzymes are widely used in cell dissociation. With some tissues papain has proved less damaging and more effective than other proteases. Lam found that of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have been isolated from adult salamander retina with papain. Huettner and Baughman described a method using papain to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Finkbeiner and Stevens applied the Huettner and Baughman method to the dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons.
The Worthington Papain Dissociation System is a set of reagents intended for use in the tissue dissociation method of Huettner and Baughman. The materials are designed for convenience and simplicity and are useful to the occasional user as well as the more experienced and frequent user. Each lot is use tested for performance in tissue dissociation and provides freshly prepared enzyme solutions for each dissociation.
* Text is quoted from the Worthington Enzyme Manual
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