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Neonatal Cardiomyocyte Isolation System
from Worthington Biochemical Corporation *
Most traditional methods published for isolating neonatal cardiomyocytes utilize crude and partially purified enzyme preparations such as trypsin NF 1:250 (Gross, et al. 1968) and collagenase. More recently the use of better characterized preparations of collagenase such as Worthington type 2 collagenase (CLS2) has provided better, more reproducible results. Like all crude collagenase preparations, though there can be significant lot-to-lot variations so many researchers make use of the Worthington Sampling Program to pre-screen several lots of enzyme before selecting a lot to purchase. Pre-sampling assures that the enzyme preparation ultimately purchased will be satisfactory; however, it is time consuming.
The Worthington Neonatal Cardiomyocyte Isolation System has been introduced to provide researchers with a reliable, convenient, and consistent cell isolation system. By utilizing purified rather than crude enzyme preparations, it has been possible to minimize the lot-to-lot variation. In addition, Worthington use-tests the kits by isolating cardiomyocytes from neonatal rat hearts to assure performance, reliability, and consistent yield of viable cells.
* Text is quoted from the Worthington Enzyme Manual
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