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Worthington

Tissue Dissociation

Deoxyribonuclease I from Worthington Biochemical Corporation*

Often as a result of cell damage, deoxyribonucleic acid leaks into the dissociation medium increasing viscosity and causing handling problems. Purified deoxyribonuclease is sometimes included in cell isolation procedures to digest the nucleic acids without damaging the intact cells.

* Text is quoted from the Worthington Enzyme Manual

Product Highlight: Deoxyribonuclease I

Product Name Description Activity Quantity Cat.No.
Deoxyribonuclease I, RNase & Protease Free, Solution

Code DPRFS

Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1mM calcium chloride. Source: Bovine Pancreas. Storage Conditions: Store at
2-8ºC or -20ºC.
≥2,000 Kunitz units per ml 100 un

500 un

Bulk

LS006342

LS006344

LS006348

Deoxyribonuclease I, Ribonuclease & Protease Free

Code DPRF

Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Lyophilized in vials. Each 10,000 unit vial contains 2 mg glycine, 2moles calcium, and ≥10,000 units of
DNase I. Each 2,500 unit vial contains 0.5mg glycine, 0.5moles calcium, and ≥2,500 units of DNase I. Dissolving the entire 10,000 unit vial in 5 ml, or the entire 2,500 unit vial in 1.25 ml, provides the equivalent of a 1 mg/ml solution. (ku = 1000un) Source: Bovine Pancreas. Storage Conditions: Store at
2-8ºC. PROTECT FROM MOISTURE.
≥2,000 units per mg dry weight

2500 un

10 ku

Bulk

LS006333

LS006334

LS006331
Deoxyribonuclease I

Code DPFF

Chromatographically purified. A lyophilized powder containing glycine as a stabilizer. Contains ≤ 0.0005% RNase. Source: Porcine Pancreas. Storage Conditions: Store at 2-8ºC. PROTECT FROM MOISTURE. ≥2,000 units per mg dry weight

25 ku

125 ku

Bulk

LS006330

LS006328

LS006332

Deoxyribonuclease I

Code D

Chromatographically purified. A lyophilized powder with glycine as a stabilizer. Source: Porcine Pancreas. Storage Conditions: Store at
2-8ºC. PROTECT FROM MOISTURE.
≥2,000 units per mg dry weight 5 mg

20 mg

100 mg

Bulk

LS002004

LS002006

LS002007

LS002009

Deoxyribonuclease I, Filtered

Code DCLS

Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity. Source: Porcine Pancreas. Storage Conditions: Store at 2-8ºC. PROTECT FROM MOISTURE. ≥2,000 units per mg dry weight 11 mg

25 mg

LS002058

LS002060

Deoxyribonuclease I, Standard Vial

Code DSV

Lyophilized in vials for assay standardization. Labeled to show established activity. Not suitable for assays at neutral pH. Source: Porcine Pancreas. Storage Conditions: Store at 2-8ºC. ~2,000 Kunitz units per vial 2 ku

5x2 ku

LS002173

LS002172

Deoxyribonuclease I

Code DP

Partially purified. A lyophilized powder. Source: Porcine Pancreas. Storage Conditions: Store at
2-8ºC.
≥2,000 units per mg dry weight 25 mg

100 mg

1 gm

Bulk

LS002138

LS002139

LS002140

LS002141

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