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Worthington

Tissue Dissociation

Chymotrypsin from Worthington Biochemical Corporation*

Chymotrypsin is a protease which preferentially catalyzes the hydrolysis of peptide bonds involving the aromatic amino acids tyrosine, phenylalanine, and tryptophan. In addition it acts upon the peptide bonds of leucyl, methionyl, asparagenyl and glutamyl residues, and the amides and esters of susceptible amino acids.

Chymotrypsin is used to a limited extent in tissue dissociation, usually in combination with trypsin and elastase.

* Text is quoted from the Worthington Enzyme Manual

Product Name Description Activity Quantity Cat.No.
Chymotrypsin, Alpha, TLCK Treated, Sequencing Grade

Code CDSEQ

 3X crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed to remove autolysis products and low molecular weight contaminants. Supplied as lyophilized powder. Source: Bovine Pancreas. Storage Conditions: Store at 2-8°C. ≥45 units per mg protein 4 x 25 µg

4 x 100 µg

 LS02130

LS02132
Chymotrypsin, Alpha, TLCK Treated

Code CDTLCK

 3X crystallized and treated with 1-chloro-3-tosylamido-7-amino-2-heptanone (TLCK) to inhibit trypsin activity [Shaw, et al., Biochemistry, 4, 2219 (1965)]. Dialyzed against 1 mM HCl to remove autolysis products and low molecular weight contaminants. Supplied as a dialyzed, lyophilized powder. Source: Bovine Pancreas. Storage Conditions: Store at
2-8°C.		 ≥45 units per mg protein 25 mg

100 mg

1 gm

Bulk

 LS001430

LS001432

LS001434

LS001438
Chymotrypsin, Alpha, Purified

Code CDS

 Chromatographically prepared by the procedure of Yapel, et al., J. Amer. Chem. Soc., 88, 2573 (1966). A lyophilized powder. Source: Bovine Pancreas. Storage Conditions: Store at 2-8°C. ≥45 units per mg protein

100 mg

1 gm

Bulk

 LS001475

LS001479

LS001477
Chymotrypsin, Alpha, 3X

Code CDI

 3X crystallized alpha chymotrypsin which is an activation product of a 3X crystallized zymogen. Dialyzed against 1mM HCl and lyophilized. Source: Bovine Pancreas. Storage Conditions: Store at
2-8°C.		 ≥45 units per mg protein 250 mg

1 gm

10 gm

Bulk

 LS001448

LS001450

LS001451

LS001453
Chymotrypsin, Alpha, 1X

Code CDAG

 1X crystallized as zymogen and activated. Dialyzed against 1mM HCl and lyophilized. Source: Bovine Pancreas. Storage Conditions: Store at
2-8°C.		 ≥35 units per mg protein

1 gm

10 gm

Bulk

 LS001333

LS001334

LS001332
Chymotrypsinogen A, Purified

Code CGC

 5X crystallized, electrophoretically homogeneous. Supplied as a dialyzed, lyophilized powder. Intrinsic activity ≤ 0.25 u/mg chymotrypsin. Source: Bovine Pancreas. Storage Conditions: Store at
2-8°C.		 Activates to ≥45 units per mg protein

1 gm

5 gm

Bulk

 LS005630

LS005623

LS005622

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